Identification and molecular characterization of a novel flavin-free NADPH preferred azoreductase encoded by azoB in Pigmentiphaga kullae K24

Table 1 Summary of purification of azoreductase from the recombinant E. coli^a

Step	Total protein (mg)	Total activity (U)*	Specific activity (U/mg)	Yield (%)
Crude cell-extract	258	750.5	2.9	100%
Phenyl Sepharose FF	57.6	570.0	9.9	76%
HiPrep SP XL	33.2	334.3	10.1	45%

ISSN: 1471-2091