Figure 4

Grouping of 30 azoreductases or hypothetic azoreductases with conserved dinucleotide binding domain. The phylogenetic tree, inferred using the Neighbor-Joining method, shows the relative position of AzoB among other azoreductases. Bootstrap values for 100 replicates are given. Protein sources and GenBank accession numbers are as follows. AzoA (AAR38851)_Enterococcus faecalis; AzoR (A4W2Z7)_Streptococcus suis 98HAH33; AzoR2 (Q9CIH9)_Lactococcus lactis subsp. lactis; LMHCC_1847 (YP_002350802)_Listeria monocytogenes HCC23; AzrA (AB263756)_Bacillus sp. B29; AcpD (YP_252301)_Staphylococcus haemolyticus JCSC1435; AcpD (YP_039668)_Staphylococcus aureus subsp. aureus MRSA252; AcpD (YP_149218)_Geobacillus kaustophilus HTA426; YvaB (NP_391234)_Bacillus subtilis subsp. subtilis strain 168; YvaB (YP_001422634)_Bacillus amyloliquefaciens FZB42; AzoR (AAG04174)_Pseudomonas aeruginosa PAO1; AZOR (Q8X9S9)_Escherichia coli O157:H7; AzoR (1V4B_A)_Escherichia coli; Azo1 (AY545994)_Staphylococcus aureus ATCC 25923; Azo1 (Q4L3N6)_Staphylococcus haemolyticus JCSC1435; SERP0206 (YP_187802)_Staphylococcus epidermidis RP62A; AZR (ACF54629)_Staphylococcus cohnii AZR; L8106_10307 (ZP_01622489)_Lyngbya sp. PCC 8106; GYMC10DRAFT_0416 (ZP_03036583)_Geobacillus sp. Y412MC10;Azr (AB032601)_Bacillus sp. OY1-2; YB0110 (AY150311)_Rhodobacter sphaeroides; AZR (AB071368)_Bacillus subtilis ISW1214; AZR (AB071367)_Geobacillus stearothermophilus IFO13737; AzoB (GQ247527)_Pigmentiphaga Kullae K24; AzoA k24 (AY165002)_Pigmentiphaga Kullae K24; EU307209 (ACA34616)_Erwinia chrysanthemi; RSMK02502 (YP_002254667)_Ralstonia solanacearum MolK2; H16_A2352 (YP_726815)_Ralstonia eutropha H16; RALTA_A1896 (YP_002005902)_Cupriavidus taiwanensis strain LMG 19424; AzoB (AF466104)_Xenophilus azovorans KF46F.