Figure 2From: Identification and molecular characterization of a novel flavin-free NADPH preferred azoreductase encoded by azoB in Pigmentiphaga kullae K24Purification of P. kullae K24 AzoB from the recombinant E. coli. Lane M, protein size markers; lane 1, extraction of the recombinant E. coli; lane 2, ammonium sulfate deposition suspension; lane 3, pooled fractions with phenyl Sepharose FF; lane 4, fraction of concentrated sample of the pooled fractions with phenyl Sepharose FF; lane 5, pooled fractions with HiPrep SP XL.Back to article page