Cloning and characterization of Escherichia coli DUF299: a bifunctional ADP-dependent kinase - Pi-dependent pyrophosphorylase from bacteria

Table 1 ADP/ATP-dependent inactivation of bacterially-expressed E. coli PEPS by bacterially-expressed E. coli PSRP.

Reaction mixtures	PEPS activity at zero time (%)	PEPS activity after 5 min (%)	PEPS activity after 10 min (%)
Complete	100	16	4
Minus ADP/ATP	100	98	103
Minus PSRP	100	97	98
Minus PEPS	0	0	0
Complete plus 5 mM pyruvate	100	95	94

PEPS inactivation was measured as described in Materials and methods. The experiment was repeated five times and results presented in the table are from one experiment that was representative of all five experiments.

ISSN: 1471-2091