Figure 3
Effect of PEP pre-treatment on the ADP-dependent inactivation of PEPS. Purified PEPS was incubated with PEP (as shown by the square brackets) and the ATP removed by Sephadex G25 gel chromatography. The PEPS was then incubated in the presence of 25 mM Hepes-KOH, 5 mM MgCl2, 5 mM DTT at pH 8.0. Additions as indicated were 2 mM ADP, 2 mM glucose (Glu), 5 units of hexokinase (HK) and 0.1 mM ATP. Experiments were conducted at least three times and the results presented in this figure are representative of the results obtained.