Figure 4
From: alpha-Sarcin catalytic activity is not required for cytotoxicity
A) Colocalization of α-sarcin with ribosomal marker RPS6. HeLa cells were transfected with α-sarcin-mychis cDNA (wt) and after 24 hours, fixed and labeled with anti-myc (FITC channel) and anti-RPS6 (rhodamine channel). (Bar 10 μM) B) Localization of the R121Q-mychis tagged mutant form of α-sarcin. The localization of both mutants appeared identical to wild-type α-sarcin. (Bar 10 μM) C) α-sarcin, and the R121Q and H137Q mutants induce apoptosis. Cos7 cells were transfected with pcDNA3, α-sarcin wildtype (wt), α-sarcin R121Q, or α-sarcin H137Q. After 24 hrs cells were collected and analyzed for annexinV-FITC and propidium iodide staining using FACS.