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Table 6 Effects of different inhibitors (two selected peptides F1 and F2, tannic acid, cibacron blue, hematin, and ethacrynic) on the GST activity.

From: A novel method for screening the glutathione transferase inhibitors

Inhibitor (1 μM)

Specific GST activity (units/mg)

 

CDNB (1 mM)

DCNB (1 mM)

Control

2.80 ± 0.01 (100%)

4.51 ± 0.02 (100%)

F1

1.20 ± 0.01(43.1%)

2.65 ± 0.02 (58.8)

F2

0.73 ± 0.01 (26.1%)

1.49 ± 0.02 (33.2%)

tannic acid

0.16 ± 0.01 (5.6%)

0.49 ± 0.01 (10.9%)

cibacron blue

0.51 ± 0.01 (18.3%)

0.96 ± 0.01 (21.3%)

hematin

0.99 ± 0.01 (35.2%)

1.90 ± 0.02 (42.1%)

ethacrynic acid

1.54 ± 0.01 (55.1%)

3.66 ± 0.02 (81.2%)

  1. Experiments were performed using glutathione and CDNB or DCNB as substrates. The activity was determined as described in the Methods section. Mean values ± SD for triplicate determinations are shown. A unit of GST activity was defined as the formation of 1 μmol of the product per min under the conditions of the specific assay. Specific activity is defined as the units of enzyme activity per mg of GST in the reaction system.
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BMC Biochemistry

ISSN: 1471-2091

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