Figure 1

Cloning all GST gene fragments into the plasmid DNA vector with the covering all gene fragments (CAGF) cloning method. A): The gene fragments of GST, B): The amplification of GST fragments using the system containing ddNTP, which can terminate the amplification reaction, and produce the DNA sequences with the single base differences, thus, the reaction system can produce a large library of fragments with single base differences. C): The binding of amplified products, D): Digestion of the CAGF cloning products with Exonuclease VII to form the blunt-ended DNA fragments. E): Amplification of the whole pFliTrx plasmid with the primers FP2 and RP2, F): The linearized pFilTrx plasmid was linked with the DNA library of the gene encoding GST.