Dictyostelium cells bind a secreted autocrine factor that represses cell proliferation

BMC Biochemistry

Table 3 The measured K_D and Bmax for the binding of rAprA to vegetative cells.

Cell type	K_D, μg/ml	K_D, nM	Bmax, ng/5 × 10⁵ cells	Bmax, molecules/cell
WT	0.16 ± 0.05	2.6 ± 0.8	3.1 ± 0.4	62,000 ± 8,000
*aprA* ^-	0.03 ± 0.02	0.5 ± 0.3	4.6 ± 0.6	92,000 ± 11,000
*cfaD* ^-	0.04 ± 0.03	0.7 ± 0.5	4.5 ± 0.7	91,000 ± 14,000
*crlA* ^-	0.11 ± 0.04	1.9 ± 0.6	3.3 ± 0.3	66,000 ± 7,000

For each cell line, the K_D in μg/ml and the Bmax in ng/5 × 10⁵ cells was obtained from the curve fits in Figure 6. The K_D in nM and Bmax in molecules/cell were calculated using a molecular mass of 60 kDa for rAprA. All values are mean ± SEM, n = 3. For both the the K_D's and the Bmax's, the difference between any two cell lines is not significant (p > 0.05, 1-way ANOVA, using either Tukey's or Bonferroni's test).

ISSN: 1471-2091