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Figure 5 | BMC Biochemistry

Figure 5

From: Dictyostelium cells bind a secreted autocrine factor that represses cell proliferation

Figure 5

Time course of rAprA binding to cells. Cells of the indicated strains (WT is wild-type) were incubated with 150 ng/ml of rAprA for the indicated times at 4°C. Cells were collected and the bound myc-tagged rAprA was quantitated by western blots (staining for the myc tag), using known amounts of rAprA as standards. The plot symbols are the same as those in Figure 2. Values are mean ± SEM (n = 3). Lines are curve fits of an association binding curve; the dashed line is the fit to aprA-.

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