Figure 2

rAprA slows cell proliferation. A) Different concentrations of rAprA were added to the indicated cell types, and after 12 hours cells were collected and counted. WT is wild-type. Values are mean ± SEM (n = 3). The absence of error bars indicates that the error was smaller than the plot symbol. The lines are sigmoidal dose response curve fits to the data; the dashed line is the fit to aprA^-. The inhibition of wild type and aprA^- proliferation is significant with p < 0.01 at 0.1 μg/ml and higher rAprA concentrations (1-way ANOVA, Dunnett's test). B) A 2:1 mixture of rAprA and rCfaD was added to wild-type cells as in A above, so that at, for instance, 0.32 μg/ml rAprA there was an additional 0.16 μg/ml rCfaD. Values are mean ± SEM (n = 3). The line is a sigmoidal dose response curve fit to the data. The inhibition of wild type proliferation is significant with p < 0.05 at 0.02 μg/ml rAprA/0.01 μg/ml rCfaD and higher concentrations (t test).