Figure 1
From: Dictyostelium cells bind a secreted autocrine factor that represses cell proliferation
The concentration of extracellular AprA increases with cell density. A) An SDS-polyacrylamide gel of recombinant AprA (left lane) and molecular mass standards (right lane) was stained with Coommassie.B) Using different concentrations or recombinant AprA to make a standard curve, Western blots were used to determine the extracellular concentration of AprA as a function of cell density. Values are mean ± SEM (n = 3). The absence of error bars indicates that the error was smaller than the plot symbol.