Table 1 Identification of novel ERα phosphorylation sites.
Peptide | Amino acid | MED Cycle | Candidate phosphopeptides | Identity | Verified by mutagenesis | Phospho- antibody |
|---|---|---|---|---|---|---|
A | Serine | 5 | 278- GEVG (S) AGDMR -287 | Serine 282 | + | + |
B | Serine | 4 | 556- GGA (S) VEETDQSHLATAGSTSSHSLQK -581 | Serine 559 | + | + |
C | Serine | 7 | 172-GSMAME (S) AK-180 288- AANLWP (S) PLMIK -299 450-SIILLN(S) GVYTFLSSTLK- 467 521-GMEHLY(S) MK- 529 | Serine 294 | + | + |
D | Serine | 10 | 38- PLGEVYLDS (S) KP AVYNYPEGAAYEFNAAAA ANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSV( S ) P( S ) PLMLLHPPPQL ( S ) PFLQPHGQQVPYYLENE PSGYTVR -142 184-YCAVCNDY (S) GYHYGVWSCEGCK-206 | Serine 47 | + | - |