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Figure 2 | BMC Biochemistry

Figure 2

From: The acidic domains of the Toc159 chloroplast preprotein receptor family are intrinsically disordered protein domains

Figure 2

Expression and purification of recombinant A-domains of atToc159 and atToc132. (A) N-terminally His6-tagged versions of atToc132 (132AHis) and atToc159 (159AHis) were expressed in E. coli, purified using Ni2+-NTA chromatography (Ni2+, lanes 2 & 5) and ion exchange (IE, lanes 3 & 6), and analyzed using SDS-PAGE stained with Coomassie blue. Molecular weight markers (kD) are indicated (lanes 1 & 4). (B) Identity of the purified proteins was confirmed by Western blotting with antibodies against the A-domains of atToc132 (α-132A) and atToc159 (α-159A).

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BMC Biochemistry

ISSN: 1471-2091

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