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Figure 4 | BMC Biochemistry

Figure 4

From: Comparison of the receptor FGFRL1 from sea urchins and humans illustrates evolution of a zinc binding motif in the intracellular domain

Figure 4

Interaction of the C-terminal domain from FGFRL1 with zinc and nickel ions. GST fusion proteins were mixed with nickel or zinc beads, washed and eluted with 1.2 M imidazole. The eluted proteins were resolved on a polyacrylamide gel, transferred to a nitrocellulose membrane and stained with antibodies against GST. Specifically eluted proteins are compared to the starting material (Input). The fusion proteins comprised amino acid residues 400-471 (Human U), 472-504 (Human L) and 492-532 (Sea Urchin L).

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BMC Biochemistry

ISSN: 1471-2091

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