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Table 2 Peptide modulation of supplemented -and pre-incubated PMCA assay

From: Prion protein self-peptides modulate prion interactions and conversion

  

complemented PMCA-assay

  

pre-incubated PMCA-assay

  

peptide

m.e.1

median

s.e.m.

n2

sign.3

median

s.e.m.

n2

sign.3

NTG

1000

2.07

0.24

3

 

4.15

0.60

5

 
 

5000

3.56

0.73

3

*

5.91

0.76

5

**

 

25000

4.68

1.26

3

**

4.13

0.63

5

 

OR

500

6.26

1.27

4

***

§n.t.

   
 

1000

4.61

0.87

5

***

3.85

0.54

4

 
 

5000

2.74

0.68

4

 

4.44

0.85

4

 
 

25000

2.57

0.33

2

 

3.99

1.19

4

 

TD2

1000

3.71

0.73

8

n.q.

1.73

0.35

4

 
 

5000

3.00

0.93

8

 

3.15

0.33

4

**

 

25000

4.63

0.62

8

**

2.91

0.34

4

*

AM

1000

1.92

0.27

3

 

4.36

0.28

7

 
 

5000

4.03

0.34

3

**

5.38

0.76

7

*

 

25000

2.36

0.46

3

 

5.63

0.50

7

**

CO

1000

1.56

0.02

2

 

8.59

0.46

5

***

 

5000

2.02

0.58

2

 

4.95

1.43

5

 
 

25000

1.97

0.22

2

 

6.09

1.53

5

*

CT

1000

1.80

0.21

2

 

6.21

0.39

5

**

 

5000

1.41

0.29

2

 

5.23

1.76

5

 
 

25000

2.85

1.12

2

 

4.77

0.92

5

 
  1. 1 amount of molar excess of peptide compared to PrPC tested in the PMCA assay
  2. 2 numbers of independent measurements performed
  3. 3 comparison of conversion ratios of the peptide supplemented -or pre-incubated PMCA and their corresponding standards using the unpaired Student's t-test (p-values < 0.05 are considered statistically different). Significantly different values are marked; p-values between 0.05 and 0.001 with *, p-values between 0.001 and 0.0001 with ** and p-values ≤ 0.0001 with ***. The p-value of a comparison that was not quite significantly different is marked with n.q.
  4. §n.t. = not tested