Figure 4

Effects of peptide pre-incubation on PrP^res formation in ovine PMCA. Bar graphs depicting PrP^res formation of a standard PMCA reaction compared to a PMCA reaction in which the scrapie negative homogenate was pre-incubated with peptide with different molar excess amounts of peptide NTG (A), peptide OR (B), peptide TD2 (C), peptide AM (D), peptide CO (E) and peptide CT (F) before adding scrapie positive homogenate. For each peptide its specific corresponding standard is depicted (grey bar). The number of independent measurements (n), the median and s.e.m. for each peptide and molar excess are summarized in Table 2. An unpaired Student's t-test was performed to determine whether the PrP^res formation with peptide was significantly different to the corresponding standard PrP^res formation. P-values are listed in Table 2 and significant differences are marked; p-values between 0.05 and 0.001 with *, p-values between 0.001 and 0.0001 with ** and p-values ≤ 0.0001 with ***.