Figure 3

Effects of peptide supplementation on PrP^res formation in ovine PMCA. Bar graphs depicting PrP^res formation of a standard PMCA reaction compared to a PMCA reaction supplemented with different molar excess amounts of peptide NTG (A), peptide OR (B), peptide TD2 (C), peptide AM (D), peptide CO (E) and peptide CT (F). For each peptide its specific corresponding standard is depicted (grey bar). In order to determine the optimal molar excess of octarepeat peptide OR in the supplemented PMCA, a single test with peptide OR added at molar excess 1, 10, 100 and 250 was performed. The amounts of PrP^res in these reactions were comparable to the standard. As an example results at molar excess 1 and 100 are depicted in graph B. The number of independent measurements (n), the median and s.e.m. for each peptide and molar excess are summarized in Table 2. An unpaired Student's t-test was performed to determine whether PrP^res formation with peptide was significantly different to the corresponding standard PrP^res formation. P-values are listed in Table 2 and significant differences are marked; p-values between 0.05 and 0.001 with *, p-values between 0.001 and 0.0001 with ** and p-values = 0.0001 with ***.