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Table 7 Reactivation of pCMB treated CPRHG by sulfhydryl reagents

From: Purification and characterization of cysteine protease from germinating cotyledons of horse gram

Substance added

Concentration

(mM)

Relative activity

(%)

Control

-

100

pCMB

10

0

pCMB + β-ME

5

10

53

77

pCMB + DTT

5

10

73

75

pCMB +Glutathione

5

10

85

104

pCMB + Cysteine

5

10

71

83

  1. The hydrolyzing activity is expressed as per cent of control (19.73 units/h/mg enzyme)