Skip to main content

Table 4 Effect of various metal ions on purified CPRHG

From: Purification and characterization of cysteine protease from germinating cotyledons of horse gram

 

Relative activity (%)

Reagent

Conc. of reagents (mM)

 

0.5

1.0

2.0

5.0

10

NiCl2

81

86

68

63

57

CoCl2

73

76

63

57

56

Cupric acetate

19

13

11

7

4

Manganese chloride

101

101

111

136

158

Lead acetate

89

90

89

64

34

Zinc acetate

58

35

20

8

2

Magnesium Chloride

116

102

102

102

95

Mercuric Chloride

20

15

7

3

0

Control

100

100

100

100

100

  1. The hydrolyzing activity is expressed as per cent of control (19.73 units/h/mg enzyme)