Table 3 Hydrolysis of protein substrates by purified CPRHG
From: Purification and characterization of cysteine protease from germinating cotyledons of horse gram
Substrate | Conc. of substrate | pH | Reaction rate (mmoles of amino acids released/hr/mg) |
|---|---|---|---|
Horse gram seed proteins | 1% | 5.5 | 612.34 |
BSA | 1% | 5.2 | 126.60 |
Casein | 1% | 5.5 | 132.16 |
Gelatin | 1% | 5.5 | 220.80 |
Hemoglobin | 1% | 4.0 | 106.04 |
CBZ-L- Phenyl alanine | 2 mM | 5.5 | Nil |
Leu-p-nitroanilide | 2 mM | 5.6 | Nil |