Table 1 Summary of the purification of CPRHG from germinating horse gram seeds
From: Purification and characterization of cysteine protease from germinating cotyledons of horse gram
Purification step | Total Protein (mg) | Total activity (Units/hr) | Specific activity (Units/mg protein) | Yield (%) Enzyme activity | Purification fold |
|---|---|---|---|---|---|
Crude extract | 9405.00 | 1578.00 | 0.168 | 100.00 | 1.00 |
Ammonium sulfate precipitation (30 - 60%) | 2713.00 | 1187.00 | 0.437 | 75.22 | 2.44 |
DEAE-cellulose | 130.80 | 628.00 | 4.801 | 40.00 | 28.27 |
CM-Sephacel | 3.65 | 72.58 | 19.88 | 4.59 | 118.36 |