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Figure 4 | BMC Biochemistry

Figure 4

From: Biosynthesis of the proteasome inhibitor syringolin A: the ureido group joining two amino acids originates from bicarbonate

Figure 4

13C-NMR spectra of in vivo NaH13CO 3 -labeled and unlabeled syringolin A. The spectra of NaH13CO3-labeled (A) and unlabeled (B) syringolin A have been scaled to give equal signal intensities for the methyl groups of the valine residues (17.4, 17.5, 19.0, and 19.1 ppm shifts). The signals at 157.8 ppm (marked 1; clipped off) and 132.8 ppm (marked 2) correspond to the ureido carbonyl group and the lysine C4 position, respectively. DMSO, DMSO solvent signal. C. Scaled-down version of part of the spectra given in (A) and (B) to show the difference in signal intensity of the ureido carbonyl group in NaH13CO3-labeled and unlabeled syringolin A, respectively.

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