Skip to main content
Figure 2 | BMC Biochemistry

Figure 2

From: Biosynthesis of the proteasome inhibitor syringolin A: the ureido group joining two amino acids originates from bicarbonate

Figure 2

Gel blot analysis of Pir7b transcript accumulation. A. RNA was extracted from rice leaves infiltrated with water (C), Pss B301D-R (WT), a sylA plasmid insertion mutant (sylA_KO), a syl cluster deletion mutant (Δsyl), and Δsyl (pPL3syl), the deletion mutant complemented with the wild-type syl gene cluster. Top panel, autoradiogram (exposed for 5 h); bottom panel, ethidium bromide (EtBr)-stained agarose gel. B. Lanes were loaded with RNA extracted from rice leaves infiltrated as indicated. C, water control; B301D-R, Pss wild-type strain; P.p, P. putida P3; P.p. (pPL3syl), P. putida P3 transformed with the syl gene cluster; SM, Pss SM; SM (pPL3syl); Pss SM transformed with pPL3syl, and syringolin A solutions of the indicated concentrations. Top panel, autoradiogram (exposure times indicated on top), bottom panel, EtBr-stained gel.

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us