Figure 5
From: Biochemical characterization of malate synthase G of P. aeruginosa
Determination of the kinetic parameters of malate synthase G of P. aeruginosa. The formulas for the calculation of the kinetic parameters are indicated. In the formulas, subscript A and B refer to glyoxylate and acetyl CoA, respectively. (A) The primary Lineweaver-Burk plots show reciprocal velocity (minutes/nmol) in function of the reciprocal glyoxylate concentration (in μM-1). Each line represents a concentration of acetyl CoA: 200 (black diamond), 100 (black square), 50 (black triangle), 25 (black X), 15 μM (black dot). R2 of the trendlines are 0.995, 0.999, 0.989, 0.996 and 0.982, respectively. (B) The secondary plot gives the reciprocal apparent velocities (in minutes/nmol) as a function of the reciprocal acetyl CoA concentration (in μM-1). The R2 of the trendline is 0.9781.