Figure 9
From: Human Rad51 mediated DNA unwinding is facilitated by conditions that favour Rad51-dsDNA aggregation
Effect of KCl on RAD51 binding to dsDNA: gel shift assay. Supercoiled ĻX174 DNA (30 Ī¼M) was incubated with RAD51 (3 Ī¼M) with ATP (1 mM) in binding buffer R at 37Ā°C for 12 minutes in presence of increasing concentrations of KCl [0 mM, 25 mM, 50 mM, 100 mM, 140 mM & 200 mM] (lanes 2ā7), followed by gel-shift analyses (Methods). Lane 1 represents no protein control.