Figure 6
From: Human Rad51 mediated DNA unwinding is facilitated by conditions that favour Rad51-dsDNA aggregation
RAD52 promotes coaggregation of RAD52-RAD51-DNA complexes: centrifugation assay followed by DNA analysis. Centrifugation assay was carried out as explained in legend for Fig. 5. Panels (D-H) correspond to same samples described in Fig. 5, except that samples were deproteinized for DNA analyses (Methods). Controls: Lanes s and p of Panels A, B & C correspond to supernatant and pellet fractions for no proteins, RAD52 (2 Ī¼M) and [RAD51 (3 Ī¼M) plus RAD52 (2 Ī¼M)] controls respectively (all containing DNA without any nucleotide cofactors). Percentage of DNA (relaxed and unwound forms) in pellet fraction was expressed as a ratio of the total DNA (relaxed and unwound forms) in both pellet and supernatant fractions and plotted as a function of RAD52 concentration (Panel I).