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Figure 4 | BMC Biochemistry

Figure 4

From: Human Rad51 mediated DNA unwinding is facilitated by conditions that favour Rad51-dsDNA aggregation

Figure 4

RAD52 promotes formation of large complexes of RAD52-RAD51-DNA: gel shift assay. Supercoiled Ļ†X174 DNA (30 Ī¼M) was incubated with increasing concentrations of RAD51 (3 Ī¼M, 6 Ī¼M & 9 Ī¼M) either in presence or absence of RAD52 (2 Ī¼M) in binding buffer R at 37Ā°C for 12 minutes, in presence of either ATP, ADP, ATPĪ³S, AMP-PNP, or AMP (1 mM each) or no nucleotide, followed by agarose gel assay (Methods). Controls (Lane 1 in Panel A: no proteins; Panel D: no RAD51). Lane 5 in Panel A was excised (from the region shown in thatched box) and subjected to analyses on 12% SDS-PAGE gel followed by Coomassie staining (lane 1, Panel E). Purified RAD51 (lane 2, Panel E) and RAD52 proteins (lanes 3, Panel E).

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