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Table 4 Mutation distribution of rpoB in λDE3-containing E. coli CC102 (mutM+mutY+) harboring pET11a and pET11a-V45A.

From: Insights into the role of Val45 and Gln182 of Escherichia coli MutY in DNA substrate binding and specificity

Plasmid

No. of clones with rpoB mutation (%)

 

pET11a

pET11a-V45A

A:T → T:A

30 (81.1)a

19 (61.3)

G:C → A:T

5 (13.5)

12 (38.7)

G:C → T:A

1 (2.7)

0 (0)

A:T → G:C

1 (2.7)

0 (0)

Total

37

31

  1. aThe number in parenthesis are the percentages of each mutation type.