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Figure 3 | BMC Biochemistry

Figure 3

From: DNA binding kinetics of two response regulators, PlnC and PlnD, from the bacteriocin regulon of Lactobacillus plantarum C11

Figure 3

Binding of f-PlnC and f-PlnD to P plnA . The sensor response curves show the interaction between f-PlnC (a) or f-PlnD (b) and P plnA at protein concentrations of 50, 100, 250, 500 and 750 nM. Association was allowed to proceed for 120 seconds at 25°C with subsequent dissociation by injecting running buffer for 180 seconds. All curves were corrected for bulk refractive index change and non-specific binding to the control DNA before estimating the binding kinetics.

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