Figure 2From: DNA binding kinetics of two response regulators, PlnC and PlnD, from the bacteriocin regulon of Lactobacillus plantarum C11SPR analysis of the interaction between f-PlnC or f-PlnD and the regulated promoters in the C11- pln. f-PlnC (a) and f-PlnD (b) were allowed to associate to the promoters P plnA , P plnM , P plnG , P plnE and P plnJ for 120 seconds at 25°C followed by injection of running buffer for 180 seconds for dissociation. All curves were corrected for bulk refractive index change and non-specific binding to the control DNA. The regulators were injected to the flow cells at a concentration of 1000 nM.Back to article page