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Figure 6 | BMC Biochemistry

Figure 6

From: TIPT2 and geminin interact with basal transcription factors to synergize in transcriptional regulation

Figure 6

Geminin and TIPT2 in the chromatin of TATA box-containing and TATA-less promoter regions. ChIP assays were performed for promoter regions and the intergenic region as indicated, using the U2OS cell line stably producing HA-TIPT2 (HA-TIPT8). (A) Western blot analysis of HA-TIPT2 protein from different stably transfected clones in comparison with endogenous TIPT2 and Lamin B. (B) ChIP assay. The acetylated H3 was used as positive control. The chromatin was immunoprecipitated with normal mouse IgG (IgGm) as negative control for anti-TBP, or with normal rabbit IgG (IgGr) for anti-acetylated H3, anti-HA, and anti-geminin, respectively.

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BMC Biochemistry

ISSN: 1471-2091

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