Figure 4
Geminin cooperates with TIPT2 and TBP to activate TATA box-containing promoters. (A) Activation of the AdMLP by the indicated combinations of TIPT2, TIPT2m, TBP, TBPL1 and geminin. (B) Activation of the AdMLP by TBP, TIPT2, and geminin or geminin mutants. Gmnn m2 lacks the coiled-coil domain, Gmnn m3 lacks the Brg1 binding domain. (C) Activation of the reporters pGL3-AdMLP, pGL3-AdMLPm1 (mutated TATA box), and pGL3-AdMLPm3 (mutated BREuelement) by the indicated combinations of TBP, TIPT2, and geminin. For sequence motifs see Figure 3E. (D) Activation of the TK promoter by the indicated combinations of TBP, TIPT2, and geminin. (E) Activation of the TK promoter by the indicated combinations of TBP, TBPL1, TIPT2, and geminin. (F) Activation of the TK promoter by the indicated combinations of TBP, TIPT2, and geminin, in the presence of siRNA against geminin. (G) Knock-down of endogenous geminin protein by transfection of U2OS cells with siRNA. The level of Lamin B protein indicates the gel loading of the protein extracts.