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Figure 7 | BMC Biochemistry

Figure 7

From: A novel human NatA Nα-terminal acetyltransferase complex: hNaa16p-hNaa10p (hNat2-hArd1)

Figure 7

hNaa16p co-sediments with polysomal fractions in a salt sensitive manner. Polysomal pellets from HeLa cells expressing hNaa16p-FLAG were resuspended in buffer containing increasing concentrations of KCl. Cell lysate (L), supernatant post first ultracentrifugation (S) and polysomal pellets after KCl treatment were analyzed on SDS-PAGE/Western blotting. The membrane was incubated with anti-FLAG, anti-hNaa15p, anti-hNaa10p, anti-L26 (ribosomal protein) and anti-CytC antibodies. Molecular-mass markers (in kDa) are indicated on the left hand side. Results shown are representative of three independent experiments.

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