Figure 5
From: A novel human NatA Nα-terminal acetyltransferase complex: hNaa16p-hNaa10p (hNat2-hArd1)
Expression of h NAA15 and h NAA16 in human cell lines. (A) The expression of hNAA15 and hNAA16 mRNA in 10 different human cell lines was analysed by real-time quantitative PCR (RT-qPCR) using gene specific primers. Values were normalized to the expression of RpLP2. An independent normalization was performed using a second reference gene, RPol2, producing almost identical results (data not shown). The mean ± SD of triplicate experiments was calculated. HepG2, hepatocellular carcinoma; HEK293, kidney adenocarcinoma; HCT116, colon carcinoma; A431, epidermoid carcinoma; SK-MEL-2, malignant melanoma; NCI-H1299, non-small cell lung carcinoma; GaMG, glioblastoma; SK-N-MC, neuroepithelioma; MCF7, breast adenocarcinoma; HeLa, cervix adenocarcinoma. (B) As (A) using 10 different human cell lines originating from the thyroid gland. TAD-2/Nthy ori 3.1, primary human thyroid cells; CGHT-W-1, follicular thyroid carcinoma; NPA/B-CPAP/ONCO-DG-1/BHT-101, papillary thyroid carcinoma; 8305C/CAL-62/ARO, anaplastic thyroid carcinoma.