Figure 10
From: A novel human NatA Nα-terminal acetyltransferase complex: hNaa16p-hNaa10p (hNat2-hArd1)
Knockdown of h NAA16 reduces cell viability. (A) HeLa cells were transfected with 50 nM unspecific, hNAA15 or hNAA16 siRNAs. Cell viability was determined by WST-1 assay 72 hours post transfection. The viability of the control cells transfected with Ctr siRNA was set to 100%. The mean ± SD of three parallel experiments was calculated. (B) HeLa cells were transfected with 50 nM unspecific, hNAA15 or hNAA16 siRNAs for 72 hours and analyzed by phase contrast microscopy (PH) to observe cell morphology and Hoechst33342 staining to visualize nuclei.