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Figure 6 | BMC Biochemistry

Figure 6

From: The endocannabinoid anandamide is a precursor for the signaling lipid N-arachidonoyl glycine by two distinct pathways

Figure 6

Comparison of NAGly production in C6 glioma cells after incubation with deuterium-labeled arachidonic acid (D 8 AA) or ethanolamine moiety deuterium-labeled AEA (D 4 AEA). The products measured were arachidonoyl chain deuterium-labeled NAGly (D8NG); non-deuterium-labeled NAGly (D0NG); and glycine moiety deuterium-labeled NAGly (D2NG). + denotes an addition of the compound to the cell media for 1 hour before lipid extraction. -denotes compounds that were not present during the incubation. # p ≤ 0.05 compared to levels of D8NG in D8AA treatment group; * p ≤ 0.05 compared to levels of D2NG in D4AEA+ URB 597 treatment group; n = 6–8 per group.

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