Figure 5
Chromatograms of lipid extracts of C6 Glioma of the tandem mass spectrometric (MS) methods for both deuterium-labeled NAGly (D 2 NAGly) in which the parent mass in negative mode [362.3]- is paired fragment mass of deuterium-labeled glycine [76.0]- and the non-deuterium-labeled N -arachidonoyl glycine (D 0 NAGly) that has a parent mass in negative ion mode of [360.3]- and a paired fragment mass of glycine [74.0]-. A) Chromatogram of MS scans for D2NAGly and D0NAGly from the lipid extract of C6 Glioma cells that were incubated with DMSO followed by D4AEA. B) Chromatogram of MS scans for D2NAGly and D0NAGly from the lipid extract of C6 Glioma cells that were incubated with 1 μM URB 597 followed by D4AEA.