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Figure 2 | BMC Biochemistry

Figure 2

From: The endocannabinoid anandamide is a precursor for the signaling lipid N-arachidonoyl glycine by two distinct pathways

Figure 2

Chromatograms of the tandem mass spectrometric (MS) method for deuterium-labeled NAGly (D 8 NAGly) in which the parent mass in negative mode [368.3]- is paired fragment mass of glycine [74.0]-. A) Chromatogram of an MS scan for the 368.3/74 pair from RAW 267.4 cell extracts that were incubated with deuterium-labeled anandamide. B) Chromatogram of a scan for the 368.3/74 pair with the synthesized D8NAGly standard (std). C) Overlay of the two independent scans.

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