Table 3 Structure of N-linked carbohydrates in bovine TAFI
From: Biochemical characterization of bovine plasma thrombin-activatable fibrinolysis inhibitor (TAFI)
Site | N-Glycans/Occupancy** | Observed glycopeptide mass (Da) | Theoretical glycopeptide mass (Da) | Glycan mass (Da) | Glycans*** |
|---|---|---|---|---|---|
N 22 | Yes/100% | 3157.2 | 3157.4 | 1622.6 | Gal2Man3GlcNAc4 |
3448.3 | 3448.5 | 1913.7 | Neu5Ac1Gal2Man3GlcNAc4 | ||
3464.3 | 3464.5 | 1929.7 | Neu5Gc1Gal2Man3GlcNAc4 | ||
3739.5 | 3739.6 | 2204.8 | Neu5Ac2Gal2Man3GlcNAc4 | ||
3755.5 | 3755.6 | 2220.8 | Neu5Gc1Neu5Ac1Gal2Man3GlcNAc4 | ||
4030.6 | 4030.7 | 2495.9 | Neu5Ac3Gal2Man3GlcNAc4 | ||
4046.7 | 4046.7 | 2511.9 | Neu5Gc1Neu5Ac2Gal2Man3GlcNAc4 | ||
4062.6 | 4062.7 | 2527.9 | Neu5Gc2Neu5Ac1Gal2Man3GlcNAc4 | ||
4321.8 | 4321.8 | 2787.0 | Neu5Ac4Gal2Man3GlcNAc4 | ||
4337.8 | 4337.8 | 2803.0 | Neu5Gc1Neu5Ac3Gal2Man3GlcNAc4 | ||
4353.8 | 4353.8 | 2819.0 | Neu5Gc2Neu5Ac2Gal2Man3GlcNAc4 | ||
N 51 | Yes/100% | 3533.6 | 3533.5 | 1622.6 | Gal2Man3GlcNAc4 |
3824.7 | 3824.6 | 1913.7 | Neu5Ac1Gal2Man3GlcNAc4 | ||
3840.7 | 3840.6 | 1929.7 | Neu5Gc1Gal2Man3GlcNAc4 | ||
4115.9 | 4115.7 | 2204.8 | Neu5Ac2Gal2Man3GlcNAc4 | ||
4131.9 | 4131.7 | 2220.8 | Neu5Gc1Neu5Ac1Gal2Man3GlcNAc4 | ||
4407.1 | 4406.8 | 2495.9 | Neu5Ac3Gal2Man3GlcNAc4 | ||
4423.1 | 4422.8 | 2511.9 | Neu5Gc1Neu5Ac2Gal2Man3GlcNAc4 | ||
4439.1 | 4438.8 | 2527.9 | Neu5Gc2Neu5Ac1Gal2Man3GlcNAc4 | ||
N 63 | Yes/100% | 2389.9 | 2390.0 | 1622.6 | Gal2Man3GlcNAc4 |
2681.0 | 2681.1 | 1913.7 | Neu5Ac1Gal2Man3GlcNAc4 | ||
2697.0 | 2697.1 | 1929.7 | Neu5Gc1Gal2Man3GlcNAc4 | ||
2972.1 | 2972.2 | 2204.8 | Neu5Ac2Gal2Man3GlcNAc4 | ||
2988.0 | 2988.2 | 2220.8 | Neu5Gc1Neu5Ac1Gal2Man3GlcNAc4 | ||
3263.3 | 3263.3 | 2495.9 | Neu5Ac3Gal2Man3GlcNAc4 | ||
3279.2 | 3279.3 | 2511.9 | Neu5Gc1Neu5Ac2Gal2Man3GlcNAc4 | ||
3295.3 | 3295.3 | 2527.9 | Neu5Gc2Neu5Ac1Gal2Man3GlcNAc4 | ||
N 86 | Yes/100% | 2851.0 | 2851.2* | 1622.6 | Gal2Man3GlcNAc4 |
3142.1 | 3142.3* | 1913.7 | Neu5Ac1Gal2Man3GlcNAc4 | ||
3158.1 | 3158.3* | 1929.7 | Neu5Gc1Gal2Man3GlcNAc4 | ||
3433.2 | 3433.4* | 2204.8 | Neu5Ac2Gal2Man3GlcNAc4 | ||
3449.2 | 3449.4* | 2220.8 | Neu5Gc1Neu5Ac1Gal2Man3GlcNAc4 | ||
3724.3 | 3724.5* | 2495.9 | Neu5Ac3Gal2Man3GlcNAc4 | ||
3740.4 | 3740.5* | 2511.9 | Neu5Gc1Neu5Ac2Gal2Man3GlcNAc4 | ||
3756.4 | 3756.5* | 2527.9 | Neu5Gc2Neu5Ac1Gal2Man3GlcNAc4 | ||
N 219 | unglycosylated | ||||
N 226 | unglycosylated |