Biochemical characterization of bovine plasma thrombin-activatable fibrinolysis inhibitor (TAFI)

BMC Biochemistry

Table 2 Summary of bovine TAFI activation products

Product**	Proteinase used in activation	SDS-PAGE mass (kDa)	Theoretical mass (kDa)	N-terminal sequence*
Bovine TAFI zymogen	N/A (full length TAFI)	56	46.4	FQRGVLSALP
Bovine TAFIa	trypsin solulin/thrombin	36	35.9	ASSSYYEQYH
Bovine TAFIa cut at the n-terminus	trypsin	32	29.2	AKNAMWID
Bovine TAFI activation peptide	trypsin solulin/thrombin	29	10.5	FQRGVLSALP
Bovine TAFI activation peptide	trypsin	25	NA	FQRGVLSALP
Bovine TAFIa N-terminal peptide	trypsin	<6	6.7	ASSSYYEQYH

*Determined by Edman Degradation.
**Upon cleavage of bovine TAFI by either trypsin or solulin/thrombin complex, fragments were separated by SDS-PAGE, transferred to PVDF membrane and subjected to Edman –degradation as described in the method section. The obtained n-terminal sequences of fragments and their molecular weight is listed.

ISSN: 1471-2091