Biochemical characterization of bovine plasma thrombin-activatable fibrinolysis inhibitor (TAFI)

BMC Biochemistry

Table 1 Summary of bovine and human TAFI and TAFIa kinetic values

	Human TAFI		Bovine TAFI		Human TAFIa		Bovine TAFIa
Equation*	Vmax	Km	Vmax	Km	Vmax	Km	Vmax	Km
Hanes	40.17	3.41	31.67	4.63	106.00	3.14	330.67	7.86
Eadie-Hofstee	43.00	3.96	32.50	4.65	113.33	3.68	332.00	7.84
Eisenthal-Cornish-Bowden	40.83	3.91	33.33	4.69	112.67	3.74	329.33	8.24
Hyperbolic Regression	41.33 ± 7.93	3.70 ± 1.51	33.40 ± 6.33	4.85 ± 2.50	106.67 ± 8.34	2.83 ± 0.89	326.67 ± 39.67	5.28 ± 1.96
Average values	41.33	3.75	32.73	4.71	109.67	3.35	329.67	7.31
Kcat (min^-1)	243.12		192.53		4386.80		13186.80
Kcat/Km (min^-1/mM)	64.83		40.88		1309.49		1803.94

*The values for Km and Vmax were determined by the direct fit of the Michaelis-Menten equation employing four graphical methods. The data represent the enzyme-catalysed reaction for 0.33 μM TAFI and 0.025 μM TAFIa.

ISSN: 1471-2091