Figure 4

Bovine TAFI cleaves Hip-Arg substrate. The intrinsic activity of the bovine and human protein (1 μg) was investigated by incubating TAFI with Hip-Arg substrate in the absence (A) or presence of 5 mM 1, 10-phenantroline (B) or 1 μg TCI (C). The cleaved product, hippuric acid (1), was then separated from the internal standard (2) by RP-HPLC. Bovine TAFI, similar to human TAFI, produces considerable hippuric acid, and this carboxypeptidase activity is abolished by addition of either 1, 10-phenantroline or TCI.