Figure 6
From: Exploring the functional interaction between POSH and ALIX and the relevance to HIV-1 release
POSH mediated enhancement of virus release requires a functional ALIX binding site in Gag. Virus release, Gag processing, and exogenous expression of POSH were analyzed by Western blot (upper panel). Amount of infectious units released from the cells was analyzed by β-galactosidase quantification after infection of TZM-bl cells (lower panel). Shown are infectivities relative to the HIV-1ΔPTAP control ± SD. Lane 1 shows HIV-1ΔPTAP mutant co-transfected with the empty pcDNA3.1V5His control vector, lane 2 shows HIV-1ΔPTAP mutant co-transfected with a V5-POSH expression plasmid, lanes 3 and 4 show the HIV-1ΔPTAP/ΔYP double mutant co-transfected with the empty control plasmid and the vector expressing V5-POSH, respectively.