Figure 3From: Exploring the functional interaction between POSH and ALIX and the relevance to HIV-1 releasePOSH targets multiple sites in ALIX. T7-tagged ALIX derivatives were expressed in HEK-293 cells and then subjected to immunoprecipitation with anti-T7. Isolated ALIX was subsequently incubated in an in vitro ubiquitination reaction and ALIX-ubiquitin conjugation was analyzed as described in Material and Methods and in the legend to figure 2.Back to article page