Skip to main content
Figure 1 | BMC Biochemistry

Figure 1

From: Exploring the functional interaction between POSH and ALIX and the relevance to HIV-1 release

Figure 1

POSH and ALIX are interacting proteins. A. Interaction in mammalian cells. Flag-tagged ALIX and V5-tagged POSH-were co-expressed in 293-HEK cells. ALIX immune complexes were isolated from cell detergent extracts by immunoprecipitation with anti-FLAG and analyzed for the presence of POSH by Western blot with anti-V5. B. Direct interaction in vitro. Bacterially-expressed POSH (50 ng) and ALIX (50 ng) were incubated in binding buffer as described in Materials and Methods and then subjected to immonoprecipitation with anti-FLAG. Immune complexes were subsequently analyzed for the presence of POSH by Western blot with anti-POSH (PT1).

Back to article page