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Figure 5 | BMC Biochemistry

Figure 5

From: Both Ca2+ and Zn2+ are essential for S100A12 protein oligomerization and function

Figure 5

Mass-spectrometry of S100A12/metal complexes. Bar charts showing the number of metal ions observed to be bound to the S100A12 dimers when S100A12 was dialyzed against a range of metal ion concentrations and analyzed by ESI FTICR mass spectrometry. (A) A range of Zn2+ concentrations; control was a solution with no metal ions; (B) a range of Ca2+ concentrations; (C) a range of Ca2+ concentrations in the presence of 1 μM Zn2+. All charts show the sum of the intensities for each metal ion number, summed over all charge states present. (D) ESI FTICR mass spectra of 200 μM S100A12 with no metal ions added; (E) 100 μM ZnCl2 and 200 μM CaCl2 added; (F) 200 μM ZnCl2 and 400 μM CaCl2. D = S100A12 dimers, T = S100A12 tetramers, H = S100A12 hexamers.

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