Figure 1
From: Both Ca2+ and Zn2+ are essential for S100A12 protein oligomerization and function
NSOM topography and fluorescent images of MKN74 cells treated with S100A12. in F12 (A) and RPMI 1640 (B) cell media. Major fraction of the clusters detected has an intensity of ~2 MHz (million counts per second). Fractions with intensities ~4, 8, and 10 MHz were also detected suggesting the presence of S100 aggregates greater than hexamers. Total number of clusters detected was ~500/image. (C-E) Images of gastric carcinoma MKN74 cells captured by epifluorescence microscopy. Cells were incubated in the presence of S100A12 protein in RPMI (C), F12K (D) and F12 (E) cellular mediums without serum. Cells were stained with affinity-purified rabbit anti-S100A12.