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Figure 6 | BMC Biochemistry

Figure 6

From: Redox biology of Mycobacterium tuberculosis H37Rv: protein-protein interaction between GlgB and WhiB1 involves exchange of thiol-disulfide

Figure 6

Reduction of GlgB's disulfide by reduced WhiB1. After interaction between WhiB1 and GlgB, the reduced cysteines of GlgB were trapped using AMS and the samples were resolved by 8% SDS-PAGE. Panels are: (1) resolved GlgB on gel, as visualized under UV light, the fluorescent band shows the binding of AMS to reduced cysteines of GlgB as labeled, (2) coomassie stained gel (after photographed under UV light) to show the presence of GlgB. The AMS labeled GlgB also showed mobility shift. Lanes are indicated on the top.

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BMC Biochemistry

ISSN: 1471-2091

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