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Figure 5 | BMC Biochemistry

Figure 5

From: Redox biology of Mycobacterium tuberculosis H37Rv: protein-protein interaction between GlgB and WhiB1 involves exchange of thiol-disulfide

Figure 5

Thioredoxin affinity chromatography. (A) Immuno-blot after thioredoxin affinity chromatography to show that the binary complex between GlgB and WhiB1C40S was destabilized by DTT. Panels: (1) interaction of GlgB with WhiB1C40S, (2) interaction of GlgB with WhiB1WT. (B) In-solution protein-protein interaction to show the formation of a binary complex between GlgB and WhiB1C40S. After reactions, the samples were resolved by 8–18% step gradient SDS-PAGE and stained with coomassie blue. A binary complex between WhiB1C40S and GlgB at ~97 kDa marker position (lanes 3 & 4) disappeared when β-ME was added (lanes 12 & 13). Rests of the lanes are controls as labeled. The triangles represent the interaction carried out using two different concentrations of WhiB1 and GlgB (5 or 10 μM of each protein).

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BMC Biochemistry

ISSN: 1471-2091

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